polyclonal rabbit anti sk3 antibody (Alomone Labs)
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polyclonal rabbit anti sk3 antibody
Polyclonal Rabbit Anti Sk3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sk3+antibody/Anti-KCNN3+(KCa2%2E3%2C+SK3)+(N-term)+Antibody/bio_rxiv__64898__2026__03__19__712770-252-30-35
Average 95 stars, based on 79 article reviews
Polyclonal Rabbit Anti Sk3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sk3+antibody/Anti-KCNN3+(KCa2%2E3%2C+SK3)+(N-term)+Antibody/bio_rxiv__64898__2026__03__19__712770-252-30-35
Average 95 stars, based on 79 article reviews
polyclonal rabbit anti sk3 antibody - by Bioz Stars,
2026-09
95/100 stars
Images
1) Product Images from "SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION"
Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION
Journal: bioRxiv
doi: 10.64898/2026.03.19.712770
Figure Legend Snippet: a-c Voltage traces from ventromedial interneurons of the rhythmogenic CPG region (L1-L2) recorded under control conditions ( a ), after tamapin application (10 nM, b ), or after lei-dab7 application (10 nM, c ). d Bar graph showing the proportion of interneurons displaying bursting in response to increasing concentrations of lei-dab7 (teal) or tamapin (purple). e Representative action potentials evoked by near-threshold current injections under control conditions (black), tamapin (purple), or lei-dab7 (teal). f-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying AHP amplitude ( f ), AHP duration ( g ), and firing frequency ( h ) under the three conditions. i-k Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying burst duration ( i ), amplitude ( j ), and frequency ( k ) across interneurons treated with apamin (pink), tamapin (purple), or lei-dab7 (teal). l-o Representative voltage traces from interneurons expressing control shRNA ( l ) or SK3-targeting shRNA ( m-o ), showing heterogeneous bursting phenotypes: bursts at rest/rheobase ( m ), bursts requiring stronger depolarization ( n ), and elliptic bursting dynamics ( o ). p-r Raincloud plots comparing burst duration ( p ), amplitude ( q ), and frequency ( r ) in interneurons after SK3 knockdown (orange) and after apamin application (pink). Numbers in parentheses denote recorded cells; each dot represents a single cell. Data points plotted beyond the dashed vertical line indicate values outside the axis range. n.s., not significant; * P < 0.05; ** P < 0.01; *** P < 0.001 (two-sided Fisher’s exact test for d ; Kruskal-Wallis with Dunn’s post hoc test versus control for f-h and i-k ; two-sided Mann-Whitney test for p-r ). For detailed P values, see Source Data.
Techniques Used: Control, Whisker Assay, Expressing, shRNA, Knockdown, Single Cell, MANN-WHITNEY
Figure Legend Snippet: a Representative low-magnification confocal image of GFP-expressing Hb9 interneurons in the ventromedial spinal cord. The asterisk marks the soma of an identified interneuron. The central canal (cc) is indicated by the dashed line. Scale bar, 25 µm. b High-magnification images of the soma indicated in a , showing GFP fluorescence (top), SK2 immunolabeling (middle), and a merged GFP/SK2 overlay (bottom). Dashed lines delineate the somatic membrane. Scale bar, 10 µm. c-d Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( c ) and density ( d , clusters per µm 2 ) of SK2, SK3, and co-localized SK2/3 clusters at the somatic membrane. e Confocal images of an Hb9 interneuron soma showing GFP (left), SK3 immunolabeling (middle), and a merged GFP/SK3 overlay (right). Dashed lines delineate the somatic membrane. Scale bar, 20 µm. f Confocal images of an intracellularly recorded HB9 interneuron filled with biocytin (magenta), showing GFP immunofluorescence (green), SK2 (red), and SK3 immunolabeling (cyan), alongside a merged GFP/SK3 signal. Scale bar, 20 µm. g-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( g ) and density ( h ) of SK2, SK3, and co-localized clusters along the dendrites. Numbers in parentheses denote the number of cells ( c, d ) or dendrites ( g, h ) analyzed; each dot represents a single measurement. *** P < 0.001 (two-sided unpaired t-test for c and g ; One-way ANOVA for d and h ). For detailed P values, see Source data.
Techniques Used: Expressing, Fluorescence, Immunolabeling, Membrane, Whisker Assay, Immunofluorescence
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Double Staining:Article Title: SK3/TRPC1/Orai1 complex regulates SOCE-dependent colon cancer cell migration: a novel opportunity to modulate anti-EGFR mAb action by the alkyl-lipid Ohmline Article Snippet: .. Double staining was performed by incubation first with caveolin-1, and then with Incubation:Article Title: SK3/TRPC1/Orai1 complex regulates SOCE-dependent colon cancer cell migration: a novel opportunity to modulate anti-EGFR mAb action by the alkyl-lipid Ohmline Article Snippet: .. Double staining was performed by incubation first with caveolin-1, and then with Article Title: Differential modulation of SK channel subtypes by phosphorylation. Article Snippet: Equal amounts of protein (15 ug) were separated by SDS-PAGE gel (Bio-Rad). .. The proteins were transferred to PVDF membranes and incubated overnight at 4 °C with primary GFP antibody (1:2000; Invitrogen, Lot: 2,015,993), SK1 antibody (1:1000; RayBiotech, Lot: 907,002,018), SK2 antibody (1:200; Alomone Labs, Lot: APC028AN2325), Western Blot:Article Title: GW24-e1393 The effect of Bim silencing by siRNA on apoptosis induced by hypoxia in rat cardiomyocyte Article Snippet: (70% decrease) and intracellular lipid accumulation as well as decreased expression of GRP78 and CHOP in ABCG1-deficient EC.. In addition, endothelial apoptosis and ER stress-related molecules were induced when EC were loaded with soluble cholesterol using cholesterol-loaded cyclodextrin, otherwise endothelial apoptotic response and expression of GRP78 and CHOP were suppressed when depletion of cellular cholesterol by cyclodextrin in ABCG1-deficient endothelial cells.. 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The MTT result showed that the survival rate was decreased (p< 0.05). Marker:Article Title: Differential modulation of SK channel subtypes by phosphorylation. Article Snippet: Equal amounts of protein (15 ug) were separated by SDS-PAGE gel (Bio-Rad). .. The proteins were transferred to PVDF membranes and incubated overnight at 4 °C with primary GFP antibody (1:2000; Invitrogen, Lot: 2,015,993), SK1 antibody (1:1000; RayBiotech, Lot: 907,002,018), SK2 antibody (1:200; Alomone Labs, Lot: APC028AN2325), Clinical Proteomics:Article Title: Differential modulation of SK channel subtypes by phosphorylation. Article Snippet: Equal amounts of protein (15 ug) were separated by SDS-PAGE gel (Bio-Rad). .. 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The proteins were transferred to PVDF membranes and incubated overnight at 4 °C with primary GFP antibody (1:2000; Invitrogen, Lot: 2,015,993), SK1 antibody (1:1000; RayBiotech, Lot: 907,002,018), SK2 antibody (1:200; Alomone Labs, Lot: APC028AN2325), Article Title: Calcium-activated and voltage-gated potassium channels of the pancreatic islet impart distinct and complementary roles during secretagogue induced electrical responses Article Snippet: Western blot analysis Protein extracts were prepared from human islets by extraction with SDS loading buffer (1% SDS, 30 mmol l −1 Tris-HCl, pH 6.8, 5%β-mercaptoethanol, 5% glycerol, and 0.1% bromophenol blue) and heating at 70°C for 10 min. Proteins were prepared as a Western blot on a nitrocellulose membrane (Bio-Rad Laboratories, Hercules, CA, USA). .. 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Proteins were prepared as a Western blot on a nitrocellulose membrane (Bio-Rad Laboratories, Hercules, CA, USA). .. After electrophoresis through a 12% denaturing polyacrylamide gel, Saline:Article Title: Calcium-activated and voltage-gated potassium channels of the pancreatic islet impart distinct and complementary roles during secretagogue induced electrical responses Article Snippet: Western blot analysis Protein extracts were prepared from human islets by extraction with SDS loading buffer (1% SDS, 30 mmol l −1 Tris-HCl, pH 6.8, 5%β-mercaptoethanol, 5% glycerol, and 0.1% bromophenol blue) and heating at 70°C for 10 min. Proteins were prepared as a Western blot on a nitrocellulose membrane (Bio-Rad Laboratories, Hercules, CA, USA). .. After electrophoresis through a 12% denaturing polyacrylamide gel, |
