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Alomone Labs polyclonal rabbit anti sk3 antibody

a-c Voltage traces from ventromedial interneurons of the rhythmogenic CPG region (L1-L2) recorded under control conditions ( a ), after tamapin application (10 nM, b ), or after lei-dab7 application (10 nM, c ). d Bar graph showing the proportion of interneurons displaying bursting in response to increasing concentrations of lei-dab7 (teal) or tamapin (purple). e Representative action potentials evoked by near-threshold current injections under control conditions (black), tamapin (purple), or lei-dab7 (teal). f-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying AHP amplitude ( f ), AHP duration ( g ), and firing frequency ( h ) under the three conditions. i-k Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying burst duration ( i ), amplitude ( j ), and frequency ( k ) across interneurons treated with apamin (pink), tamapin (purple), or lei-dab7 (teal). l-o Representative voltage traces from interneurons expressing control shRNA ( l ) or <t>SK3-targeting</t> shRNA ( m-o ), showing heterogeneous bursting phenotypes: bursts at rest/rheobase ( m ), bursts requiring stronger depolarization ( n ), and elliptic bursting dynamics ( o ). p-r Raincloud plots comparing burst duration ( p ), amplitude ( q ), and frequency ( r ) in interneurons after SK3 knockdown (orange) and after apamin application (pink). Numbers in parentheses denote recorded cells; each dot represents a single cell. Data points plotted beyond the dashed vertical line indicate values outside the axis range. n.s., not significant; * P < 0.05; ** P < 0.01; *** P < 0.001 (two-sided Fisher’s exact test for d ; Kruskal-Wallis with Dunn’s post hoc test versus control for f-h and i-k ; two-sided Mann-Whitney test for p-r ). For detailed P values, see Source Data.

Polyclonal Rabbit Anti Sk3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sk3+antibody/bio_rxiv__64898__2026__03__19__712770-252-30-35?v=Alomone+Labs
Average 95 stars, based on 79 article reviews

polyclonal rabbit anti sk3 antibody - by Bioz Stars, 2026-07

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1) Product Images from "SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION"

Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION

Journal: bioRxiv

doi: 10.64898/2026.03.19.712770

Figure Legend Snippet: a-c Voltage traces from ventromedial interneurons of the rhythmogenic CPG region (L1-L2) recorded under control conditions ( a ), after tamapin application (10 nM, b ), or after lei-dab7 application (10 nM, c ). d Bar graph showing the proportion of interneurons displaying bursting in response to increasing concentrations of lei-dab7 (teal) or tamapin (purple). e Representative action potentials evoked by near-threshold current injections under control conditions (black), tamapin (purple), or lei-dab7 (teal). f-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying AHP amplitude ( f ), AHP duration ( g ), and firing frequency ( h ) under the three conditions. i-k Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying burst duration ( i ), amplitude ( j ), and frequency ( k ) across interneurons treated with apamin (pink), tamapin (purple), or lei-dab7 (teal). l-o Representative voltage traces from interneurons expressing control shRNA ( l ) or SK3-targeting shRNA ( m-o ), showing heterogeneous bursting phenotypes: bursts at rest/rheobase ( m ), bursts requiring stronger depolarization ( n ), and elliptic bursting dynamics ( o ). p-r Raincloud plots comparing burst duration ( p ), amplitude ( q ), and frequency ( r ) in interneurons after SK3 knockdown (orange) and after apamin application (pink). Numbers in parentheses denote recorded cells; each dot represents a single cell. Data points plotted beyond the dashed vertical line indicate values outside the axis range. n.s., not significant; * P < 0.05; ** P < 0.01; *** P < 0.001 (two-sided Fisher’s exact test for d ; Kruskal-Wallis with Dunn’s post hoc test versus control for f-h and i-k ; two-sided Mann-Whitney test for p-r ). For detailed P values, see Source Data.

Techniques Used: Control, Whisker Assay, Expressing, shRNA, Knockdown, Single Cell, MANN-WHITNEY

a Representative low-magnification confocal image of GFP-expressing Hb9 interneurons in the ventromedial spinal cord. The asterisk marks the soma of an identified interneuron. The central canal (cc) is indicated by the dashed line. Scale bar, 25 µm. b High-magnification images of the soma indicated in a , showing GFP fluorescence (top), SK2 immunolabeling (middle), and a merged GFP/SK2 overlay (bottom). Dashed lines delineate the somatic membrane. Scale bar, 10 µm. c-d Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( c ) and density ( d , clusters per µm 2 ) of SK2, SK3, and co-localized SK2/3 clusters at the somatic membrane. e Confocal images of an Hb9 interneuron soma showing GFP (left), SK3 immunolabeling (middle), and a merged GFP/SK3 overlay (right). Dashed lines delineate the somatic membrane. Scale bar, 20 µm. f Confocal images of an intracellularly recorded HB9 interneuron filled with biocytin (magenta), showing GFP immunofluorescence (green), SK2 (red), and SK3 immunolabeling (cyan), alongside a merged GFP/SK3 signal. Scale bar, 20 µm. g-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( g ) and density ( h ) of SK2, SK3, and co-localized clusters along the dendrites. Numbers in parentheses denote the number of cells ( c, d ) or dendrites ( g, h ) analyzed; each dot represents a single measurement. *** P < 0.001 (two-sided unpaired t-test for c and g ; One-way ANOVA for d and h ). For detailed P values, see Source data.

Figure Legend Snippet: a Representative low-magnification confocal image of GFP-expressing Hb9 interneurons in the ventromedial spinal cord. The asterisk marks the soma of an identified interneuron. The central canal (cc) is indicated by the dashed line. Scale bar, 25 µm. b High-magnification images of the soma indicated in a , showing GFP fluorescence (top), SK2 immunolabeling (middle), and a merged GFP/SK2 overlay (bottom). Dashed lines delineate the somatic membrane. Scale bar, 10 µm. c-d Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( c ) and density ( d , clusters per µm 2 ) of SK2, SK3, and co-localized SK2/3 clusters at the somatic membrane. e Confocal images of an Hb9 interneuron soma showing GFP (left), SK3 immunolabeling (middle), and a merged GFP/SK3 overlay (right). Dashed lines delineate the somatic membrane. Scale bar, 20 µm. f Confocal images of an intracellularly recorded HB9 interneuron filled with biocytin (magenta), showing GFP immunofluorescence (green), SK2 (red), and SK3 immunolabeling (cyan), alongside a merged GFP/SK3 signal. Scale bar, 20 µm. g-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( g ) and density ( h ) of SK2, SK3, and co-localized clusters along the dendrites. Numbers in parentheses denote the number of cells ( c, d ) or dendrites ( g, h ) analyzed; each dot represents a single measurement. *** P < 0.001 (two-sided unpaired t-test for c and g ; One-way ANOVA for d and h ). For detailed P values, see Source data.

Techniques Used: Expressing, Fluorescence, Immunolabeling, Membrane, Whisker Assay, Immunofluorescence

Image Search Results

Journal: bioRxiv

Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION

doi: 10.64898/2026.03.19.712770

Figure Lengend Snippet: a-c Voltage traces from ventromedial interneurons of the rhythmogenic CPG region (L1-L2) recorded under control conditions ( a ), after tamapin application (10 nM, b ), or after lei-dab7 application (10 nM, c ). d Bar graph showing the proportion of interneurons displaying bursting in response to increasing concentrations of lei-dab7 (teal) or tamapin (purple). e Representative action potentials evoked by near-threshold current injections under control conditions (black), tamapin (purple), or lei-dab7 (teal). f-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying AHP amplitude ( f ), AHP duration ( g ), and firing frequency ( h ) under the three conditions. i-k Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying burst duration ( i ), amplitude ( j ), and frequency ( k ) across interneurons treated with apamin (pink), tamapin (purple), or lei-dab7 (teal). l-o Representative voltage traces from interneurons expressing control shRNA ( l ) or SK3-targeting shRNA ( m-o ), showing heterogeneous bursting phenotypes: bursts at rest/rheobase ( m ), bursts requiring stronger depolarization ( n ), and elliptic bursting dynamics ( o ). p-r Raincloud plots comparing burst duration ( p ), amplitude ( q ), and frequency ( r ) in interneurons after SK3 knockdown (orange) and after apamin application (pink). Numbers in parentheses denote recorded cells; each dot represents a single cell. Data points plotted beyond the dashed vertical line indicate values outside the axis range. n.s., not significant; * P < 0.05; ** P < 0.01; *** P < 0.001 (two-sided Fisher’s exact test for d ; Kruskal-Wallis with Dunn’s post hoc test versus control for f-h and i-k ; two-sided Mann-Whitney test for p-r ). For detailed P values, see Source Data.

Article Snippet: Equal amounts of protein (40 μg per lane) were separated on 4-15% gradient SDS-PAGE stain-free gels (Bio-Rad), transferred to nitrocellulose membranes, and probed overnight at 4 °C with either a polyclonal rabbit anti-SK3 antibody (1:500, Alomone Labs, APC-025) or an anti-actin antibody (1:1,000, A2066, Sigma-Aldrich) in Tris-buffered saline containing 5% fat-free milk.

Techniques: Control, Whisker Assay, Expressing, shRNA, Knockdown, Single Cell, MANN-WHITNEY

Journal: bioRxiv

Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION

doi: 10.64898/2026.03.19.712770

Figure Lengend Snippet: a Representative low-magnification confocal image of GFP-expressing Hb9 interneurons in the ventromedial spinal cord. The asterisk marks the soma of an identified interneuron. The central canal (cc) is indicated by the dashed line. Scale bar, 25 µm. b High-magnification images of the soma indicated in a , showing GFP fluorescence (top), SK2 immunolabeling (middle), and a merged GFP/SK2 overlay (bottom). Dashed lines delineate the somatic membrane. Scale bar, 10 µm. c-d Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( c ) and density ( d , clusters per µm 2 ) of SK2, SK3, and co-localized SK2/3 clusters at the somatic membrane. e Confocal images of an Hb9 interneuron soma showing GFP (left), SK3 immunolabeling (middle), and a merged GFP/SK3 overlay (right). Dashed lines delineate the somatic membrane. Scale bar, 20 µm. f Confocal images of an intracellularly recorded HB9 interneuron filled with biocytin (magenta), showing GFP immunofluorescence (green), SK2 (red), and SK3 immunolabeling (cyan), alongside a merged GFP/SK3 signal. Scale bar, 20 µm. g-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( g ) and density ( h ) of SK2, SK3, and co-localized clusters along the dendrites. Numbers in parentheses denote the number of cells ( c, d ) or dendrites ( g, h ) analyzed; each dot represents a single measurement. *** P < 0.001 (two-sided unpaired t-test for c and g ; One-way ANOVA for d and h ). For detailed P values, see Source data.

Techniques: Expressing, Fluorescence, Immunolabeling, Membrane, Whisker Assay, Immunofluorescence

Examination of eplet mismatch immunogenicity in mixed lymphocyte reactions The association between the eplet risk score (ERS) and the activation status of responder CD4 + T cells was analyzed. Representative scattergrams are shown in (A), and aggregated scatterplots are shown in (B). Each stimulator-responder pair was analyzed with 3–6 biological replicates. The effect of an anti-55PP antibody (Ab) or an anti-45EV Ab on the percentage of CFSE low CD4 + T cells in the MLR was assessed using a stimulator-responder pair (pair #4, see ). Representative scattergrams are shown in (C), and aggregated data are shown in (D) and (E). Each antibody concentration group was analyzed with 4–12 biological replicates. ∗ p < 0.05. p values were determined using the Wilcoxon rank-sum test, which compared the value at 0 ng/mL Ab with those at 1 × 10 2 and 1 × 10 3 ng/mL Ab. EMn, the number of eplet mismatches; FSC, forward scatter; stim, stimulator. In the scatterplot shown in (B), large dots represent the median value of each stimulator-responder pair, small dots represent values obtained from each individual experiment, vertical lines represent ranges, and the gray area represents 95% confidence interval (CI) of the regression line. In the bar charts shown in (D) and (E), the top of the bar represents the median value, whereas the points represent values from individual experiments.

Journal: Cell Reports Medicine

Article Title: Cross-organ hierarchy of HLA molecular mismatches in donor-specific antibody development in solid organ transplantations

doi: 10.1016/j.xcrm.2025.102153

Figure Lengend Snippet: Examination of eplet mismatch immunogenicity in mixed lymphocyte reactions The association between the eplet risk score (ERS) and the activation status of responder CD4 + T cells was analyzed. Representative scattergrams are shown in (A), and aggregated scatterplots are shown in (B). Each stimulator-responder pair was analyzed with 3–6 biological replicates. The effect of an anti-55PP antibody (Ab) or an anti-45EV Ab on the percentage of CFSE low CD4 + T cells in the MLR was assessed using a stimulator-responder pair (pair #4, see ). Representative scattergrams are shown in (C), and aggregated data are shown in (D) and (E). Each antibody concentration group was analyzed with 4–12 biological replicates. ∗ p < 0.05. p values were determined using the Wilcoxon rank-sum test, which compared the value at 0 ng/mL Ab with those at 1 × 10 2 and 1 × 10 3 ng/mL Ab. EMn, the number of eplet mismatches; FSC, forward scatter; stim, stimulator. In the scatterplot shown in (B), large dots represent the median value of each stimulator-responder pair, small dots represent values obtained from each individual experiment, vertical lines represent ranges, and the gray area represents 95% confidence interval (CI) of the regression line. In the bar charts shown in (D) and (E), the top of the bar represents the median value, whereas the points represent values from individual experiments.

Article Snippet: Anti-CD4 PE-Cy7 antibody (clone SK3) , Becton, Dickinson and Company , Cat#348789; RRID: AB_400379.

Techniques: Immunopeptidomics, Activation Assay, Concentration Assay

Single cell RNAseq profiling of human ADRB1 antigen-specific CD4 + T cell responses. ( A ) Experiment design: PBMCs of human subjects expressing the DRB1*13 HLA allele were stimulated in vitro with ADRB1 167-182 peptide for 14 days. Donors showing the highest fold response in AIM T cells (OX40 + 4-1BB + ) were selected for scRNA/ TCR seq analysis. The ‘dotted line highlight T cell subsets selectively expanded by the antigen. ( B ) UMAP plot displays 12 clusters annotated from CD4 + T cells derived from control and ADRB1-stimulated conditions. ( C ) Violin plots of the log-normalized expression values of FOXP3, IL10, KLRB1, IFNG, IL5 and CSF2 per cluster (left and central panel) and the CITE-seq signal for CD69 and CD45RA (right panel). ( D ) Jitter plots show cell distributions for each cluster defined in 1B, split by subjects (S1-S5) and experimental conditions (IL-2 control or ADRB1 peptide). Dashed squares highlight the most expanded clusters in ADRB1-stimulated condition for each subject.

Journal: medRxiv

Article Title: Tracking antigen-specific T cell responses in patients with myocardial infarction

doi: 10.1101/2025.06.05.25328514

Figure Lengend Snippet: Single cell RNAseq profiling of human ADRB1 antigen-specific CD4 + T cell responses. ( A ) Experiment design: PBMCs of human subjects expressing the DRB1*13 HLA allele were stimulated in vitro with ADRB1 167-182 peptide for 14 days. Donors showing the highest fold response in AIM T cells (OX40 + 4-1BB + ) were selected for scRNA/ TCR seq analysis. The ‘dotted line highlight T cell subsets selectively expanded by the antigen. ( B ) UMAP plot displays 12 clusters annotated from CD4 + T cells derived from control and ADRB1-stimulated conditions. ( C ) Violin plots of the log-normalized expression values of FOXP3, IL10, KLRB1, IFNG, IL5 and CSF2 per cluster (left and central panel) and the CITE-seq signal for CD69 and CD45RA (right panel). ( D ) Jitter plots show cell distributions for each cluster defined in 1B, split by subjects (S1-S5) and experimental conditions (IL-2 control or ADRB1 peptide). Dashed squares highlight the most expanded clusters in ADRB1-stimulated condition for each subject.

Article Snippet: Next, cells were washed with FACS buffer and resuspended in 50 μL of antibody master mix, containing: anti-CD3 eFluor450 (clone UCHT1, ThermoFisher), - CD45 BUV496 (clone HI30, ThermoFisher), -CD4 Superbright 600 (clone SK3, ThermoFisher), -CCR7 (CD197) PerCP/Cy5.5 (clone G043H7, BioLegend), - CD45RA PE BUV737 (clone HI100, ThermoFisher), -CD25 FITC (clone M- A251, BioLegend), -CD127 PE-Cy7 (clone eBioRDR5, ThermoFisher), - CXCR3 BV480 (clone CXCR3-173, BD Biosciences), -CCR6 (CD196) BV650 (clone G034E3, BioLegend), -PD-1 PE-eFluor 610 (clone J105, ThermoFisher), -CD278 (ICOS) BUV395 (clone C398.4A /RUO, BD Biosciences), -CD69 PE-Fire640 (clone FN50, BioLegend), -CD134 (OX40) BV785 (clone Ber-ACT35/ACT35, BioLegend), -4-1BB PE (clone 4B4-1, ThermoFisher) and Human TruStain FcXTM (1:200, BioLegend).

Techniques: Expressing, In Vitro, Derivative Assay, Control

( A ) Gating strategy and ( B ) sorting experiment design. PBMCs from five subjects (S1-S5) were kept for 14 days in culture in the presence of either IL-2 or IL-2 combined with ADRB1 peptides (10 conditions). Each condition was stained with a different hashtag antibody along with CITE-seq for CD69, CD25, CD45RA and CD45RO. Singlets, live, CD3 + CD4 + OX40 +/- 4-1BB + events were sorted and cells from all conditions were pooled and sequenced as a single library. Following data filtration, 9,379 single cells were obtained.

Journal: medRxiv

Article Title: Tracking antigen-specific T cell responses in patients with myocardial infarction

doi: 10.1101/2025.06.05.25328514

Figure Lengend Snippet: ( A ) Gating strategy and ( B ) sorting experiment design. PBMCs from five subjects (S1-S5) were kept for 14 days in culture in the presence of either IL-2 or IL-2 combined with ADRB1 peptides (10 conditions). Each condition was stained with a different hashtag antibody along with CITE-seq for CD69, CD25, CD45RA and CD45RO. Singlets, live, CD3 + CD4 + OX40 +/- 4-1BB + events were sorted and cells from all conditions were pooled and sequenced as a single library. Following data filtration, 9,379 single cells were obtained.

Techniques: Staining, Filtration

HLADRB1*13 allele as a potential risk factor for ADRB1 specific T cell responses post MI. ( A ) Donut charts depicting HLA-DRB1*13 + allele frequency in healthy population and MI patients. Statistical analysis was performed with Chi-square test followed by Yates’ correction ( P =0.1988). P -values < 0.05 were considered statistically significant. ( B ) Donut charts depicting HLADRB1*13 + carrier frequency in healthy population and MI patients. Statistical analysis was performed with Fisher’s exact test (P= 0.1327). P -values < 0.05 were considered statistically significant. ( C ) Experimental workflow (left panel) and diversity index of TCRβ chain from the bulkRNAseq data of DRB1*13 + (n=7) versus DRB1*13 − (n=19) MI patients. Both groups displayed similar Troponin levels. Data are shown as mean±SEM; statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( D ) Heatmap depicting the enrichment of TCRβ sequences from in the CDR3s of MI/ DRB1*13 + , ADRB1 stimulated PBMCs (from Hapke et al. ) and MI/ DRB1*13 − bulkRNAseq data, without selection for expansion. ( E ) Heatmap depicting the core motif overlapping (>50 clones, ≥1e-04) within TCRβ sequences from and MI/ DRB1*13 + , ADRB1 stimulated PBMCs and MI/ DRB1*13 − . ( F ) Representative flow cytometry plot of CD4/Tetramer gated on live CD45 + /CD3 + /CD4 + cells (left), ( G ) Histogram (right panel) and quantitative analysis of 4-1BB + cells in tetramer + versus tetramer − cells for patient (n=8) (left panel). Data are shown as mean ± SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( H ) Representative flow cytometry plot of CD45RA/CCR7 gated on live CD45 + /CD3 + /CD4 + cells and ( I ) quantitative analysis of Naïve and central-memory T cells (T cm ) in tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean ± SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( J ) Representative flow cytometry plot of CD25/CD127 gated on live CD45 + /CD3 + /CD4 + cells (left panel) and ( K ) quantitative analysis of Tregs in tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean ± SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant.

Journal: medRxiv

Article Title: Tracking antigen-specific T cell responses in patients with myocardial infarction

doi: 10.1101/2025.06.05.25328514

Figure Lengend Snippet: HLADRB1*13 allele as a potential risk factor for ADRB1 specific T cell responses post MI. ( A ) Donut charts depicting HLA-DRB1*13 + allele frequency in healthy population and MI patients. Statistical analysis was performed with Chi-square test followed by Yates’ correction ( P =0.1988). P -values < 0.05 were considered statistically significant. ( B ) Donut charts depicting HLADRB1*13 + carrier frequency in healthy population and MI patients. Statistical analysis was performed with Fisher’s exact test (P= 0.1327). P -values < 0.05 were considered statistically significant. ( C ) Experimental workflow (left panel) and diversity index of TCRβ chain from the bulkRNAseq data of DRB1*13 + (n=7) versus DRB1*13 − (n=19) MI patients. Both groups displayed similar Troponin levels. Data are shown as mean±SEM; statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( D ) Heatmap depicting the enrichment of TCRβ sequences from in the CDR3s of MI/ DRB1*13 + , ADRB1 stimulated PBMCs (from Hapke et al. ) and MI/ DRB1*13 − bulkRNAseq data, without selection for expansion. ( E ) Heatmap depicting the core motif overlapping (>50 clones, ≥1e-04) within TCRβ sequences from and MI/ DRB1*13 + , ADRB1 stimulated PBMCs and MI/ DRB1*13 − . ( F ) Representative flow cytometry plot of CD4/Tetramer gated on live CD45 + /CD3 + /CD4 + cells (left), ( G ) Histogram (right panel) and quantitative analysis of 4-1BB + cells in tetramer + versus tetramer − cells for patient (n=8) (left panel). Data are shown as mean ± SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( H ) Representative flow cytometry plot of CD45RA/CCR7 gated on live CD45 + /CD3 + /CD4 + cells and ( I ) quantitative analysis of Naïve and central-memory T cells (T cm ) in tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean ± SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( J ) Representative flow cytometry plot of CD25/CD127 gated on live CD45 + /CD3 + /CD4 + cells (left panel) and ( K ) quantitative analysis of Tregs in tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean ± SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant.

Techniques: Selection, Clone Assay, Flow Cytometry

( A ) Gating strategy to analyse tetramer + and tetramer − cells originated from MI DRB1*13 + patients. ( B ) Backgating of tetramer + CD4 + T cells. ( C ), ( D ) Gating strategy to further analyse tetramer + and tetramer − cells.

Journal: medRxiv

Article Title: Tracking antigen-specific T cell responses in patients with myocardial infarction

doi: 10.1101/2025.06.05.25328514

Figure Lengend Snippet: ( A ) Gating strategy to analyse tetramer + and tetramer − cells originated from MI DRB1*13 + patients. ( B ) Backgating of tetramer + CD4 + T cells. ( C ), ( D ) Gating strategy to further analyse tetramer + and tetramer − cells.

Techniques:

( A ) Representative histogram (left) and quantitative analysis (right) of OX40 + tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( B ) Representative histogram (left) and quantitative analysis (right) of PD-1 + tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( C ) Representative histogram (left) and quantitative analysis (right) of Th1 (CXCR3 + ) Tetramer + versus Tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( D ) Representative histogram (left) and quantitative analysis (right) of T H 17 (CCR6 + ) tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( E ) Representative flow cytometry plot of CD45RA/CCR7 gated on live CD45 + /CD3 + /CD4 + cells and ( F ) quantitative analysis of T EMRA and T em cells in tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant.

Journal: medRxiv

Article Title: Tracking antigen-specific T cell responses in patients with myocardial infarction

doi: 10.1101/2025.06.05.25328514

Figure Lengend Snippet: ( A ) Representative histogram (left) and quantitative analysis (right) of OX40 + tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( B ) Representative histogram (left) and quantitative analysis (right) of PD-1 + tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( C ) Representative histogram (left) and quantitative analysis (right) of Th1 (CXCR3 + ) Tetramer + versus Tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( D ) Representative histogram (left) and quantitative analysis (right) of T H 17 (CCR6 + ) tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant. ( E ) Representative flow cytometry plot of CD45RA/CCR7 gated on live CD45 + /CD3 + /CD4 + cells and ( F ) quantitative analysis of T EMRA and T em cells in tetramer + versus tetramer − cells for each patient (n=8). Data are shown as mean±SEM; Statistical analysis was performed with unpaired t-test. P -values < 0.05 were considered statistically significant.

Techniques: Flow Cytometry

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